Class Enzyme Technology

Provide integrated training in the field of Applied Biocatalysis, emphasizing: the various applications of enzymes at the industrial level; the particular requirements of those applications; and the platforms that allow its implementation, namely formulation of enzymes and enzymatic reactors

Not applicable

Introduction to enzyme technology and representative exemples of applications. Enzyme structure and function. Activity and Stability of Enzymes. Principles of enzyme kinetics. Models of inhibition, denaturation and inactivation of enzymatic activity. Enzyme modification: chemical methods and genetic manipulation / protein engineering. Enzymatic formulations. Methods and supports for immobilizing enzymes. Effect of immobilization on conformation, apparent kinetics and stability of the biocatalyst. Biocatalysis in non-conventional media. Selection strategies of the reaction system. Reactors for free and immobilized biocatalysts. Fundamentals of design and operation analysis of enzymatic reactors. Strategies for selecting the type of reactor. Laboratory classes: based on model systems, the activity and stability of various enzyme formulations are compared, the influence of operational conditions on these parameters and the implementation in reactors in selected cases is addressed

Use of active methodologies, in particular provision of material prior to the class through platforms such as Moodle, with basic guidance on the subject. The referred materials include videos, audios, infographics, among others. Later in class, students ask questions, discuss the subject and compare answers Presentation and discussion of case studies, problem solving, individually or in groups, to encourage interaction and team spirit Evaluation Continuous evaluation P1: average of 2 tests or global frequency (GF) during the semester (80%) + work (20%):60% of the final grade P2: reports, behavior in class and discussion: 40% of the final grade Minimum passing grade for P1 and P2: 10 points in each: P1: minimum of 10 points (average of tests/GF) Failing approval by continuous assessment, the student can take an exam in the resit assessment period according to the assessment regulation in force. Minimum grade to pass: 10

Bhaskar, A., Vidhya, V.G. (2021) Enzyme Technology. India: Mjp Publisher. ISBN-13 ‏ : ‎ 978-8180940019 Bennett, A. (2018) Enzyme Kinetics And Regulation. United Kingdom: ED-Tech Press. ISBN:978-1-83947-176-6