Class Molecular Biology

In the field of biology, biochemistry and scientific research in general, molecular biology increasingly occupies a fundamental role. The great evolution of molecular knowledge and the development of laboratory techniques overlooking this area, become vital in areas as important as that of medicine. Basic knowledge in molecular biology will allow students to integrate in an easy way in any laboratory environment.

Not applicable

Part 1. Genome analysis 1. Genomes organization. Sequencing techniques: Sanger versus NGS (Next generation sequencing) 2. Molecular mechanisms of gene expression regulation 3. Molecular mechanisms of genetic recombination 4. Processes of genetic transfer and recombination in prokaryotes Part 2. Genomics analysis 5. Usage of genomic analysis techniques 6. Techniques for analysis of transcription process. Real time PCR vs RNAseq. 7. Alteration of gene expression - Epigenetics. DNA methylation. Histone modification and non-coding RNA (ncRNA). 8. Recombinant DNA technology Part 3. Fundamental cellular processes 9. Cellular signalling 10. Carcinogenesis   Practical program 1. Basic rules of lab security, solution preparation and pipeting. 2. Basic techniques of nucleic acid manipulation 3. Construction of restriction maps: DNA digestion with restriction enzymes and result analysis. 4. PCR: Experiments preparation and optimization. 5. Recombinant DNA molecules construction: cloning process in bacteria

In addition to the weekly follow-up, students will have at their disposal in the e-learning moodle platform, discussion topics, additional articles that may be of interest to their training. It is intended in this way to create a great involvement and active participation of students in the CU, and a daily interest for the themes discussed. Lectures will be taught in person or by elearning using the Zoom platform whenever necessary.

- Alberts, Bray, Hopkin, Johnson, Lewis, Raff, Roberts and Walter (2013). Essential Cell Biology. 4th Ed. Garland Science. - Lodish, Berk, Kaiser, Krieger, Bretscher, Ploegh, Amon and Scott (2012). Molecular Cell Biology. 7th Ed. W. H. Freeman and Company. - Tropp, B.E. (2014). Principles of Molecular Biology. Jones & Bartlett Learning. - Weaver, R. (2011). Molecular Biology. 5th Ed. McGraw-Hill Education. - Williams, S.A., Slatko, B.E., McCarrey, J.R. (2007). Laboratory Investigation in Molecular Biology. Jones & Bartlett Learning.