Class Genetic Engineering

Componente Teórica - Conhecimento dos métodos e mecanismos de modificação genética usados em Biotecnologia. São exemplos em tecnologia do DNA recombinante o uso vetores e métodos de clonagem em procariotas e eucariotas e a obtenção de animais e plantas geneticamente modificados. Componente Prática - Construção de uma biblioteca genómica de Lactobacillus plantarum num vector plasmídico, clonagem e expressão de genes, pesquisa de genes por complementação. Análise Bioinformática dos genes identificados.

Not applicable

Enzymes to modify DNA and restriction enzymes. Cloning vectors: Plasmids, Phagemids, Cosmids, BAC, YAC. Genomic and cDNA libraries. Analysis of cloned genes. DNA sequencing. Directed mutagenesis and knockout. Protein engineering. DNA manipulation in microorganisms, plants and animals. Cloning in bacteria and fungus. Strategies to transfer genes to animal cells, bacterias and plants Inducible expression systems. Recombinant induction systems. Site-specific recombination. Production of useful molecules. Recombinant therapeutic proteins. Manipulation of metabolic pathways Gene edition Improvement of agronomic characteristics by genetic manipulation. Resistance to diseases; abiotic stress; quality improvement. Genetic manipulation to study, prevent and cure diseases. Models for human diseases. DNA vaccines. Gene therapy.

The interpretation and reading of articles that include the methodologies, developments and state-of-the-art applications. This approach is considered as an experience in a dry lab.This work will be presented to the class.

Primrose, S.B. and Twyman, R.M. Principles of Gene Manipulation and Genomics. Seventh Edition. Blackwell Publishing, (2006), Oxford, UK. Lodish, H., Berk, A., Matsudaira, P., Kaiser, C.A., Krieger, M., Scott, M.P., Zipursky, L., Darnell, J. Molecular Cell Biology.. Fifth Edition. Scientific American Books, (2010) New York, USA. Videira, A. Engenharia Genética ¿ Princípios e Aplicações. LIDEL-edições técnicas, (2015), Lisboa, Portugal