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Presentation
Presentation
In this UC are taught the mechanisms of storage, transmission and expression of genetic information as well as the mechanisms involved in the regulation of these processes. Several techniques of molecular biology as well as their applications and respective analysis and interpretation of results will be presented.
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Class from course
Class from course
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Degree | Semesters | ECTS
Degree | Semesters | ECTS
Bachelor; Master Degree | Semestral | 5
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Year | Nature | Language
Year | Nature | Language
2 | Mandatory | Português
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Code
Code
ULHT477-3910
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Prerequisites and corequisites
Prerequisites and corequisites
Not applicable
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Professional Internship
Professional Internship
Não
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Syllabus
Syllabus
1 ¿ Molecular mechanisms Historical landmarks Molecular biology biomolecules: DNA, RNA and proteins Molecular biology central dogma Prokaryotic and eukaryotic genome organization DNA replication Mutation and repair mechanisms Gene expression Gene expression regulation (and deregulation) Post-translational modifications and protein degradation mechanisms 2 ¿ Molecular biology techniques DNA extraction Molecular biology enzymes Polimerase chain reaction (PCR) Molecular cloning DNA sequencing Electrophoresis and hybridization (Southern, Northern and Western blots) Genotyping Probes and molecular markers Eukaryotic gene expression manipulation techniques DNA Microarrays
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Objectives
Objectives
After attending this CU, the student should understand the molecular mechanisms involved in genetic information storage, transmission and usage as well as its regulatory steps. The student should also know a group of molecular biology techniques, their applicability and results interpretation. Technical competences in nucleic acid handling, recombinant protein expression and data analysis interpretation and discussion should also be gained. The knowledge above mentioned is of extreme importance for the student¿s future understanding of the molecular mechanisms involved in pharmacological action and of published scientific data. Autonomy, organization, responsibility, critical analysis of research data and group cooperation are promoted in the laboratory and during the elaboration of an oral presentation of a research paper.
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Teaching methodologies and assessment
Teaching methodologies and assessment
Active learning methodologies based on the resolution of practical problems, on the exposition by the students of some concepts addressed in laboratory classes and the use of observations/results obtained in the laboratory as a starting point for the discussion of more complex concepts/topics. Fieldwork involving a visit to a research center (Institute of Molecular Medicine), as part of the week of contact with the profession, so that students can have contact with scientific research in the area of Molecular Biology.
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References
References
Lodish, H et al., (2012) Molecular Cell Biology, 7th Ed, W. H. Freeman and Co. (Informação complementar online: http://bcs.whfreeman.com/lodish7e/#t_800911____) Lewin, B., Genes X, (2011) 10th Ed, Jones & Bartlett Learning. Artigos científicos fornecidos pelo docente.
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Office Hours
Office Hours
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Mobility
Mobility
No
