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Presentation
Presentation
The aim of this Curricular Unit is to give a perspective of the interest of Molecular Biology in the Veterinary Medicine practice. The fundamentals of laboratorail technologies such as PCR (real-time PCR, multiplex PCR), RFLP, molecular markers analysis, DNA sequencing, quantification of nucleic acids, will allow individual and population analysis, diagnosis of susceptibility vs resistance to diseases, quantification of viral load and a glimpse of the power of bioinformatic analysis. Moreover, the discussion of cases related to genome manipulation, its applications in industry, and aspects related to safety, ethics and animal welfare will be covered. Such information will be provided through theoretical, practical/experimental classes, and discussion of specific clinical cases. Additionally, attendance at seminars organized by the teaching team is required.
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Class from course
Class from course
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Degree | Semesters | ECTS
Degree | Semesters | ECTS
Bachelor; Master Degree | Semestral | 2
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Year | Nature | Language
Year | Nature | Language
1 | Optional | Português
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Code
Code
ULHT478-17560
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Prerequisites and corequisites
Prerequisites and corequisites
Not applicable
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Professional Internship
Professional Internship
Não
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Syllabus
Syllabus
1. Molecular Biology: genes and their function 2. The flow of information from genes to proteins in complex organisms 3. Bioinformatics: databases and tools for genetic data analysis 4. Molecular methods for sexing birds 5. Molecular methods for individual identification at the forensic level applied to wild fauna 6. Management of populations in a human-protected environment 7. Detection of genetically modified organisms (GMO) 8. Diagnosis 9. Safety, ethics and animal welfare
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Objectives
Objectives
I. Review the basis of Molecular Biology (genes and their function) and Genetics (molecular markers, genetic analyzes of individuals and populations). II.Learn the main molecular tools available to the veterinary care providers: laboratorial methods to understand genes and their function, PCR, genomic sequencing, bioinformatics. III. Learn the use and application of these molecular tools (species identification, diagnosis, quantification of viral load, etc...) IV. Notions about animal/human safety, ethics and welfare issues
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Teaching methodologies and assessment
Teaching methodologies and assessment
During experimental classes each student, within a group , has to design an experiment that proves one piece of evidence. Various pieces of evidence will be questioned for different sets of materials and reagents. In this case, the student needs to understand the concept of the experiment and apply his/her knowledge about the functionality of cell structures or cell metabolism processes. Students are required to apply their knowledge of biochemistry, cell biology or molecular biology in an integrated way.
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References
References
Nicholas, F. W. (2009). Introduction to veterinary genetics. John Wiley & Sons. Abubakar, M. (Ed.). (2017). Trends and Advances in Veterinary Genetics. Cunha, M. V., & Silva, J. I. (2014). Abordagens Moleculares em Veterinária-Como desvendar a etiologia e a epidemiologia da infeção.
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Office Hours
Office Hours
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Mobility
Mobility
No