Class Animal cell and tissue technologies

Animal Cell and Tissue Technology is a tool of modern biotechnology involving several disciplinary areas. Culture media, facilities for the culture of animal cells and tissues and various tools for cell characterization are covered, particularly in the area of human health. This curricular unit introduces the basics of animal tissue culture as well as the historical background, culture types, maintenance and characterization tools involved in this process. Furthermore, it also focuses on animal tissue culture facilities, on biosafety guidelines for cell culture in in vitro conditions and on ways to avoid contamination.

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Description of contents Introduction to Animal Cell and Tissue Technology Animal Cells - basic concepts Cloning and expression of Heterologous proteins in animal cells Cell metabolism and its control in culture Culture media for animal cells Pos-translational modifications of the recombinant proteins Mechanisms of proliferation and cell death Bioreactors for animal cells Monitoring and control of cell cultures separation of animal cells Cell therapy and stem cells Gene therapy Tissue engineering

The teaching-learning process include theoretical explanations with interaction between teachers and students, project-based learning, flipped classrooms, and Design Thinking.   Evaluation is conducted continuously and consists of two components: theoretical component (TC) and laboratory component (LC). The TC is assessed through two midterm exams, or a comprehensive exam, and a group project. The LC assessment consists of continuous evaluation that include completion of mini-questionaries (30%), report writing (30%), a practical test (20%), and a theoretical test (20%). The final grade is calculated using a weighted average: 50% (TC) + 50% (LC), and a minimum grade of 9.5 out of 20 is required in each component for passing. This comprehensive evaluation approach ensures a balanced assessment of students' performance in both the theoretical and practical aspects of the course.   Students may opt for non-continuous evaluation, which consists of a final exam

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